After Horace Slughorn showed a cauldron of the Polyjuice Potion to his first N. The Order of the Phoenix made use of Polyjuice in 1997 in order to safely remove Harry Potter from 4 Privet Drive before his seventeenth birthday.
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The potion also played a crucial role for Harry Potter, Hermione Granger, and Ron Weasley in their second and fourth years.
It was also used in Draco Malfoy's plot to smuggle Death Eaters into Hogwarts School of Witchcraft and Wizardry in 1997, in the Battle of the Seven Potters, and in Harry, Ron and Hermione's Infiltration of the Ministry of Magic and their break-in of Gringotts Wizarding Bank in 1998. "The recipe can be found in Moste Potente Potions, which is housed in the Restricted Section of the Hogwarts library.
She also directed how the trio were to acquire the final ingredients — hair from their targets — as well as clothing to complete the disguise.
Ultimately, however, though Harry and Ron successfully assumed the appearances of Goyle and Crabbe respectively, Hermione's own attempt to take on the guise of Millicent Bulstrode went awry when Hermione used a cat hair that she had mistaken for Millicent's hair from a hair brush.
The resulting potion caused Hermione to partially transform into a cat. We’d be breaking about fifty school rules, I expect…